Facility iCal (Read Only)

Please refer to the MEGAMER facility iCal to determine open dates available for visiting scientists. Note that although the facility may be reserved for use by visitors anytime, the flow cytometer/cell sorter is only available for priority use by visiting scientist on Wednesday, Thursday and Friday of a typical work week. Weekends are available on a first come, first serve basis by either USCS researchers or visiting scientist. Still, all other equipment is considered the sole jurisdiction of a visiting researcher during their stay.

Truck iCal

Please sign up prior to using the MEGAMER truck on the Truck iCal. The truck is to be used for official business only. Ask Brandon Carter if you need clarification as to what this means. Two car keys are available. One is stored in the MEGAMER apartment on the bulletin board adjcient to the dining room table and the telephone. The other is stored in the MEGAMER lab in the office supplies drawer. A gas card is included with the key set kept in the MEGAMER lab. Visiting scientist are always given first priority use of this vehicle. Drive Safe!

StepOne qPCR iCal

Please sign up prior to using the StepOne Real Time PCR machine in the MEGAMER facility. Note that this instrument requires one to use plastics designed specifically for the StepOne. Please ask Brandon Carter should you like training on the instrument. Setup is to be conducted in the core lab space as with all other PCR preps.

Plate Reader iCal

Please refer to the Plate Reader iCal to determine open times for using the plate reader. The plate reader has the ability to run temperature sensitive experiments over an extended period of time. Should you plan to use this feature, please plan ahead. Under these circumstances I ask that you schedule time on the plate reader at least 48 hours in advance of use. Also, please use the red placard found hanging over the plate reader to let others know you are running a long term experiment. As a further precaution, please place a note on the computer asking users to avoid this computer during the duration of your experiment.

Cytometer iCal

-Individuals are required to complete a short yet comprehensive training session that will ensure that the user is able to start up and shut down the instrument and make basic troubleshooting adjustments. A summary of the startup and shut down procedures are listed here.

-Users may sign up to use the instrument via the an iCal with the following considerations:

1. Scheduling priority is given to researchers whose work is directly related to achieving the outcomes described on the MEGAMER policy page.

2. Priority on the cytometer is given to visiting scientist (nonUCSC researchers) on Wednesday, Thursday and Friday of a typical work week. UCSC researchers are given priority on Monday, Tuesday and on any date not in conflict with a visiting scientist. Weekends maybe scheduled on a first come first serve basis.

3. Visiting scientist may sign up for time on the cytometer only after their use of the MEGAMER facility has been approved.

3. Priority for UCSC researchers is given to Ocean Sciences and Institute of Marine Sciences faculty.

-Those users with greater priority (see above) are asked to reserve time on the cytometer no more than three months prior to their arrival and at least one-week in advance of use.

-Remaining available time slots must be scheduled by noon on the day before planned use. Otherwise, the instrument is available on a first come, first serve basis.

-Exceptions are always possible but must be cleared by the lab manager.